Intro

We updated our DGE analysis with fresh and new GO terms! All of these lists with identified and unidentified DEG’s are up on my account on github for your own viewing. I will list out the number of DGE’s between the treatment control and a treatment here, the number of ID’d transcripts, and the number of unidentified transcripts. At this point, the DGE’s for the treatment control, if also present in the lab control, have had pre-lab expression accounted for in the log2fold change in expression.

Foot tissue

Hypoxia treatment vs. treatment control

  • 15 genes differentially expressed
  • 7 transcripts identified
  • 8 transcripts unidentified
  • Gene of interest: Mytilus edulis NADH-ubiquinone oxidoreductase chain 2 had a 5 fold reduction in expression

Ocean acidification treatment vs. treatment control

  • 14 genes differentially expressed
  • 3 transcripts identified
  • 11 transcripts unidentified
  • Gene of interest: nothing Mytilus related

Ocean warming treatment vs treatment control

  • 32 genes differentially expressed
  • 9 transcripts identified
  • 23 transcripts unidentified
  • Gene of interest: Mytilus edulis Cytochrome c oxidase subunit 3 had a 0.017 fold reduction in expression

Gill tissue

Hypoxia treatment vs. treatment control

  • 50 genes differentially expressed
  • 7 transcripts identified
  • 43 transcripts unidentified
  • Gene of interest: Mytilus edulis NADH-ubiquinone oxidoreductase chain 2 had a 7 fold reduction in expression

Ocean acidification treatment vs. treatment control

  • 99 genes differentially expressed
  • 23 transcripts identified
  • 76 transcripts unidentified
  • Gene(s) of interest:
    • Two versions of Mytilus edulis Heavy metal-binding protein HIP, one had a 0.67795676 fold increase and the other had a 2.17770003 fold decrease
    • Mytilus edulis Metallothionein 10-IV had a 2.74 fold decrease in expression

Ocean warming treatment vs treatment control

  • 25 genes differentially expressed
  • 7 transcripts identified
  • 18 transcripts unidentified
  • Gene of interest: None Mytilus related